.jpg)
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472). Western blot was performed from the immunoprecipitate using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 pAb (AP0472) at a dilition of 1:1000. Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.
.jpg)
Immunohistochemistry of paraffin-embedded human colon using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).
.jpg)
Immunohistochemistry of paraffin-embedded rat testis using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).
.jpg)
Immunohistochemistry of paraffin-embedded mouse spinal cord using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb (AP0472) at dilution of 1:100 (40x lens).
.jpg)
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody (AP0472) at 1:1000 dilution.Jurkat cells and NIH/3T3 cells and C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb
Catalog No：AB-AP0472
Manufacturer：ABclonal
Synonyms：MAPK1/MAPK3
Applications：WB,IHC,IF,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014] |
| Immunogen: | A synthetic phosphorylated peptide around T185/Y187 of human ERK2 (NP_002736.3). |
| Gene ID: | 5594/5595 |
| Swiss prot: | P28482/P27361 |
| Molecular weight: | 36kDa/41kDa/38kDa/40kDa/43kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No：AB-AP0022
Manufacturer：ABclonal
Synonyms：ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0021
Manufacturer：ABclonal
Synonyms：ADCADN;AIM;CXXC9;DNMT;HSN1E;MCMT;m.HsaI;DNMT1
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0017
Manufacturer：ABclonal
Synonyms：CHEK1;CHK1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0013
Manufacturer：ABclonal
Synonyms：CCNE1;CCNE;pCCNE1;cyclin E1
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0005
Manufacturer：ABclonal
Synonyms：AKT2;HIHGHH;PKBB;PKBBETA;PRKBB;RAC-BETA
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
Catalog No：AB-AP0004
Manufacturer：ABclonal
Synonyms：AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1
Applications：WB,IF
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00