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Western blot analysis of extracts of various cell lines, using Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of C6 cells, using Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb (AP0974) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat fallopian tube using Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of Jurkat cells using Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb (AP0974) at dilution of 1:100 (40x lens).Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes. Blue: DAPI for nuclear staining.
Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb
Catalog No：AB-AP0974
Manufacturer：ABclonal
Synonyms：ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Erk1-T202/Y204 + Erk2-T185/Y187 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T202/Y204 of human Erk1 |
| Gene ID: | 5595/5594 |
| Swiss prot: | P27361/P28482 |
| Molecular weight: | 42kDa/44kDa |
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