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Immunohistochemistry of paraffin-embedded rat lung using Phospho-ERK1-T202 + ERK2-T185 antibody (AP0485) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using Phospho-ERK1-T202 + ERK2-T185 antibody (AP0485) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ERK1-T202 + ERK2-T185 antibody (AP0485) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) at 1:1000 dilution.Jurkat cells were treated by PMA/TPA (200 nM) at 37℃ for 10 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb (AP0485) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1S.
Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb
Catalog No：AB-AP0485
Manufacturer：ABclonal
Synonyms：H3.4;H3/g;H3FT;H3t;H3/A;H3FA;ERK1 / ERK2
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-ERK1-T202 + ERK2-T185 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185). |
| Gene ID: | 5594/5595 |
| Swiss prot: | P28482/P27361 |
| Molecular weight: | 42,44kDa |
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