Western blot analysis of extracts of 293T cells, using Phospho-EphA3-Y779 pAb (AP1056) at 1:1000 dilution or EPHA3 antibody (A8414).293T cells were treated by Pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 10s.
Phospho-EphA3-Y779 Rabbit pAb
Catalog No:AB-AP1056
Manufacturer:ABclonal
Synonyms:EK4;ETK;HEK;ETK1;HEK4;TYRO4;EPHA3
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-EphA3-Y779 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. |
Immunogen: | A phospho synthetic peptide corresponding to residues surrounding Y779 of human EphA3. |
Gene ID: | 2042 |
Swiss prot: | P29320 |
Molecular weight: | 110kDa |
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