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Western blot analysis of extracts of 293T and Hela cells, using Phospho-EphA3-Y779 antibody (AP0768) at 1:2000 dilution. 293T cells were treated by Pervanadate (1mM) for 30 minutes after serum-starvation overnight.HeLa cells were treated by Pervanadate (1nM) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-EphA3-Y779 Rabbit pAb
Catalog No：AB-AP0768
Manufacturer：ABclonal
Synonyms：EK4;ETK;HEK;ETK1;HEK4;TYRO4;EPHA3
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-EphA3-Y779 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around Y779 of human EphA3 (NP_005224.2). |
| Gene ID: | 2042 |
| Swiss prot: | P29320 |
| Molecular weight: | 60kDa/110kDa |
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