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Immunofluorescence analysis of MCF-7 cells using Phospho-ELK1-S383 antibody (AP0033). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Jurkat cells, using Phospho-ELK1-S383 antibody (AP0033) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-ELK1-S383 Rabbit pAb
Catalog No：AB-AP0033
Manufacturer：ABclonal
Synonyms：ELK1
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ELK1-S383 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. |
| Immunogen: | A synthetic phosphorylated peptide around S383 of human ELK1 (NP_005220.2). |
| Gene ID: | 2002 |
| Swiss prot: | P19419 |
| Molecular weight: | 11kDa/44kDa |
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