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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2ɑ-S51 pAb (AP0745). Western blot was performed from the immunoprecipitate using Phospho-eIF2ɑ-S51 pAb (AP0745) at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
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Western blot analysis of extracts of HeLa cells, using Phospho-eIF2ɑ-S51 pAb (AP0745) at 1:2000 dilution or EIF2S1 antibody (A0764).HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. |Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-eIF2ɑ-S51 antibody (AP0745) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-eIF2ɑ-S51 antibody (AP0745) at dilution of 1:150 (40x lens).
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Immunohistochemistry of paraffin-embedded rat spleen using Phospho-eIF2ɑ-S51 antibody (AP0745) at dilution of 1:150 (40x lens).
Phospho-eIF2α-S51 Rabbit pAb
Catalog No：AB-AP0745
Manufacturer：ABclonal
Synonyms：EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1
Applications：WB,IHC,IP
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-eIF2伪-S51 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| Immunogen: | A synthetic phosphorylated peptide around S51 of human eIF2伪 (NP_004085.1). |
| Gene ID: | 1965 |
| Swiss prot: | P05198 |
| Molecular weight: | 36kDa |
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