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Western blot analysis of extracts of various cell lines, using Phospho-eIF2ɑ-S51 Rabbit mAb (AP0692) at 1:1000 dilution.HeLa and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by TNF-ɑ (20 ng/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-eIF2α-S51 Rabbit mAb
Catalog No：AB-AP0692
Manufacturer：ABclonal
Synonyms：EIF-2;EIF-2A;EIF-2alpha;EIF2;EIF2A;EIF2S1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-eIF2伪-S51 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010] |
| Immunogen: | A synthesized peptide derived from human Phospho-EIF2S1 (S51). |
| Gene ID: | 1965 |
| Swiss prot: | P05198 |
| Molecular weight: | 36kDa |
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