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Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y992 antibody (AP0026) at 1:1000 dilution. A431 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EGFR-Y992 antibody (AP0026) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 120s.
Phospho-EGFR-Y992 Rabbit pAb
Catalog No：AB-AP0026
Manufacturer：ABclonal
Synonyms：EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-EGFR-Y992 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y992 of human EGFR. |
| Gene ID: | 1956 |
| Swiss prot: | P00533 |
| Molecular weight: | 44kDa/69kDa/77kDa/134kDa |
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