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Western blot analysis of extracts of A-431 cells, using Phospho-EGFR-Y1068 Rabbit mAb (AP0027) at 1:1000 dilution.A-431 cells were treated by EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-EGFR-Y1068 Rabbit mAb
Catalog No：AB-AP0027
Manufacturer：ABclonal
Synonyms：EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-EGFR-Y1068 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] |
| Immunogen: | A synthesized peptide derived from human Phospho-EGFR (Y1068). |
| Gene ID: | 1956 |
| Swiss prot: | P00533 |
| Molecular weight: | 170kDa |
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