.jpg)
Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (AP0845) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
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Western blot analysis of extracts of various cell lines, using Phospho-EEF2K-S366 antibody (AP0845) at 1:1000 dilution.NIH/3T3 and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 pAb (AP0845) at 1:1000 dilution or EEF2K antibody (A5404).Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 15s.
Phospho-EEF2K-S366 Rabbit pAb
Catalog No：AB-AP0845
Manufacturer：ABclonal
Synonyms：EEF2K;HSU93850;eEF-2K
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-EEF2K-S366 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. |
| Immunogen: | A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1). |
| Gene ID: | 29904 |
| Swiss prot: | O00418 |
| Molecular weight: | 82kDa |
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