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Western blot analysis of extracts of various cell lines, using Phospho-eEF2-T56 antibody (AP0832) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30uM) for 30 minutes after serum-starvation overnight. C6 cells were treated by Forskolin (10uM) for 1 hour after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-eEF2-T56 Rabbit pAb
Catalog No：AB-AP0832
Manufacturer：ABclonal
Synonyms：EEF2;EEF-2;EF-2;EF2;SCA26
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-eEF2-T56 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. |
| Immunogen: | A synthetic phosphorylated peptide around T56 of human Phospho-eEF2-T56 (NP_001952.1). |
| Gene ID: | 1938 |
| Swiss prot: | P13639 |
| Molecular weight: | 95kDa |
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