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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Cyclin E1-T77 Rabbit mAb (AP1014) at 1:1000 dilution.NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-Cyclin E1-T77 Rabbit mAb
Catalog No：AB-AP1014
Manufacturer：ABclonal
Synonyms：CCNE, pCCNE1
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Cyclin E1-T77 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. [provided by RefSeq, Apr 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T77 of human Cyclin E1 |
| Gene ID: | 898 |
| Swiss prot: | P24864 |
| Molecular weight: | 48kDa |
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