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Western blot analysis of extracts of K562 cells, using Phospho-CRK/CRKII-Y221 antibody (AP0822) at 1:1000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37℃ for 1 hour .|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-CRK/CRKII-Y221 Rabbit pAb
Catalog No：AB-AP0822
Manufacturer：ABclonal
Synonyms：CRK;CRKII;p38
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-CRK/CRKII-Y221 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. |
| Immunogen: | A synthetic phosphorylated peptide around Y221 of human CRK/CRKII (NP_058431.2). |
| Gene ID: | 1398 |
| Swiss prot: | P46108 |
| Molecular weight: | 22kDa/33kDa |
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