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Western blot analysis of extracts of various cell lines, using Phospho-CRK/CRKII-Y221 Rabbit mAb (AP1150) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-CRK/CRKII-Y221 Rabbit mAb
Catalog No：AB-AP1150
Manufacturer：ABclonal
Synonyms：CRKII, p38
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-CRK/CRKII-Y221 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y221 of human CRK/CRKII |
| Gene ID: | 1398 |
| Swiss prot: | P46108 |
| Molecular weight: | 34kDa |
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