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Western blot analysis of extracts of various cell lines, using Phospho-Chk1-S345 antibody (AP0578).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
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Western blot analysis of extracts of C6 cells, using Phospho-Chk1-S345 pAb (AP0578) at 1:1000 dilution or Chk1 antibody (A7653).C6 cells were treated by ATP(5 mM) at 30℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 5min.
Phospho-Chk1-S345 Rabbit pAb
Catalog No：AB-AP0578
Manufacturer：ABclonal
Synonyms：CHEK1;CHK1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-Chk1-S345 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S345 of human Chk1 (NP_001265.2). |
| Gene ID: | 1111 |
| Swiss prot: | O14757 |
| Molecular weight: | 43kDa/50kDa/54kDa |
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