Western blot analysis of extracts of 293T cells, using Phospho-Chk1-S296 Rabbit mAb (AP1047) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes and Doxorubicin (0.5 uM) at 37℃ for 24 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-Chk1-S296 Rabbit mAb
Catalog No:AB-AP1047
Manufacturer:ABclonal
Synonyms:CHK1
Applications:WB
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-Chk1-S296 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding S296 of human Chk1 |
Gene ID: | 1111 |
Swiss prot: | O14757 |
Molecular weight: | 54kDa |
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