Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CDK4-T172 pAb (AP0593) at 1:1000 dilution or CDK4 antibody (A0366).NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 60s.
Phospho-CDK4-T172 Rabbit pAb
Catalog No:AB-AP0593
Manufacturer:ABclonal
Synonyms:CDK4;CMM3;PSK-J3
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Product Name: | Phospho-CDK4-T172 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. |
Immunogen: | A synthetic phosphorylated peptide around T172 of human CDK4 (NP_000066.1). |
Gene ID: | 1019 |
Swiss prot: | P11802 |
Molecular weight: | 20kDa/33kDa |
Help & Support
Product
Guarantee and
Expert Support
Related products
Catalog No:AB-AP0026
Manufacturer:ABclonal
Synonyms:EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications:WB
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0025
Manufacturer:ABclonal
Synonyms:EGFR;ERBB;ERBB1;HER1;NISBD2;PIG61;mENA
Applications:WB,IP
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0012
Manufacturer:ABclonal
Synonyms:BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Applications:WB
Reactivity:Human,Rat
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0008
Manufacturer:ABclonal
Synonyms:AT1;ATA;ATC;ATD;ATDC;ATE;TEL1;TELO1;ATM
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0004
Manufacturer:ABclonal
Synonyms:AKT;CWS6;PKB;PKB-ALPHA;PRKBA;RAC;RAC-ALPHA;AKT1
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €410,00
Catalog No:AB-AP0003
Manufacturer:ABclonal
Synonyms:ABL;JTK7;p150;c-ABL;v-abl;CHDSKM;c-ABL1;ABL1;c-Abl;bcr/abl
Applications:WB,IF
Reactivity:Human,Mouse
Host:Rabbit
Conjugate:No
€65,00 – €410,00