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Western blot analysis of extracts of various cell lines, using Phospho-CDK2-T160 antibody (AP0325) at 1:1000 dilution.HeLa cells NIH/3T3 cells and C6 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-CDK2-T160 antibody (AP0325).
Phospho-CDK2-T160 Rabbit pAb
Catalog No：AB-AP0325
Manufacturer：ABclonal
Synonyms：CDK2;CDKN2;p33(CDK2)
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-CDK2-T160 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T160 of human CDK2 |
| Gene ID: | 1017 |
| Swiss prot: | P24941 |
| Molecular weight: | 30kDa/33kDa |
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