Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug Phospho-CDK1-Y15 pAb (AP0016). Western blot was performed from the immunoprecipitate using Phospho-CDK1-Y15 pAb (AP0016) at a dilition of 1:1000. HT-29 cells were treated by Serum-starvation overnight at 37℃.

Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-Y15 antibody (AP0016) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1min.

Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb (AP0016) at 1:1000 dilution or CDK1 antibody (A0220).HeLa cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours or Hydroxyurea (4 mM) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.

Immunohistochemistry of paraffin-embedded rat liver using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using Phospho-CDK1-Y15 antibody (AP0016) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of human breast cancer using Phospho-CDK1-Y15 Rabbit pAb (AP0016) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.