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Immunofluorescence analysis of MCF-7 cells using Phospho-CDK1-T14 antibody (AP0015). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-T14 antibody (AP0015) at 1:1000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10min.
Phospho-CDK1-T14 Rabbit pAb
Catalog No：AB-AP0015
Manufacturer：ABclonal
Synonyms：CDK1;CDC2;CDC28A;P34CDC2
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-CDK1-T14 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around T14 of human CDK1 (NP_001777.1). |
| Gene ID: | 983 |
| Swiss prot: | P06493 |
| Molecular weight: | 27kDa/34kDa |
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