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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-CDC25A-S76 antibody (AP1166) at 1:1000 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/ml) at 37℃ for 20 hours.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-CDC25A-S76 Rabbit pAb
Catalog No：AB-AP1166
Manufacturer：ABclonal
Synonyms：CDC25A;CDC25A2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-CDC25A-S76 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S76 of human CDC25A |
| Gene ID: | 993 |
| Swiss prot: | P30304 |
| Molecular weight: | 54kDa/59kDa |
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