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Immunofluorescence analysis of U2OS cells using Phospho-c-Myc-T58 antibody (AP0080). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded rat testis using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using P-MYC-T58 antibody (AP0080) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of 293T cells, using Phospho-c-Myc-T58 antibody (AP0080) at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-c-Myc-T58 Rabbit pAb
Catalog No：AB-AP0080
Manufacturer：ABclonal
Synonyms：MRTL;MYCC;bHLHe39;c-Myc;MYC
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-c-Myc-T58 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt’s lymphomas, suggesting its importance in the normal function of this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T58 of human MYC |
| Gene ID: | 4609 |
| Swiss prot: | P01106 |
| Molecular weight: | 48kDa/50kDa |
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