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Western blot analysis of extracts of various cell lines, using Phospho-c-Myc-S62 Rabbit mAb (AP0989) at 1:1000 dilution.293T cells were treated by Forskolin (10 uM) at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-c-Myc-S62 Rabbit mAb
Catalog No：AB-AP0989
Manufacturer：ABclonal
Synonyms：MRTL, MYCC, bHLHe39, c-Myc
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-c-Myc-S62 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S62 of human c-Myc |
| Gene ID: | 4609 |
| Swiss prot: | P01106 |
| Molecular weight: | 57kDa |
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