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Western blot analysis of extracts of various cell lines, using Phospho-c-Jun-T93 antibody (AP1218) at 1:500 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight.NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-c-Jun-T93 Rabbit pAb
Catalog No：AB-AP1218
Manufacturer：ABclonal
Synonyms：AP-1;AP1;c-Jun;JUN;c
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-c-Jun-T93 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T93 of human JUN |
| Gene ID: | 3725 |
| Swiss prot: | P05412 |
| Molecular weight: | 35kDa |
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