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Western blot analysis of extracts of various cell lines, using Phospho-c-Jun-T91 antibody (AP1216) at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
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Immunohistochemistry of paraffin-embedded rat lung using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens).
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Immunofluorescence analysis of U2OS cells using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Phospho-c-Jun-T91 Rabbit pAb
Catalog No：AB-AP1216
Manufacturer：ABclonal
Synonyms：AP-1;AP1;c-Jun;JUN;c
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-c-Jun-T91 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Immunogen: | A synthetic phosphorylated peptide around T91 of human JUN. |
| Gene ID: | 3725 |
| Swiss prot: | P05412 |
| Molecular weight: |
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