Western blot analysis of extracts of NIH/3T3 cells, using Phospho-c-Jun-T91 Rabbit mAb (AP1003) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 ug/mL) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts of 293T cells, using Phospho-c-Jun-T91 Rabbit mAb (AP1003) at 1:1000 dilution.293T cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-c-Jun-T91 Rabbit mAb
Catalog No:AB-AP1003
Manufacturer:ABclonal
Synonyms:AP-1, AP1, c-Jun
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €325,00
Product Name: | Phospho-c-Jun-T91 Rabbit mAb |
Category: | Phosphorylated Antibodies |
Description: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008] |
Immunogen: | A phospho specific peptide corresponding to residues surrounding T91 of human c-Jun |
Gene ID: | 3725 |
Swiss prot: | P05412 |
Molecular weight: | 43kDa |
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