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Immunofluorescence analysis of U2OS cells using Phospho-c-Jun-S73 antibody (AP0047). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Phospho-c-Jun-S73 antibody (AP0047) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.C6 cells were treated by UV at room temperature for 15-30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-c-Jun-S73 Rabbit pAb
Catalog No：AB-AP0047
Manufacturer：ABclonal
Synonyms：AP-1;AP1;c-Jun;JUN;c
Applications：WB,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-c-Jun-S73 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Immunogen: | A synthetic phosphorylated peptide around S73 of human c-Jun (NP_002219.1). |
| Gene ID: | 3725 |
| Swiss prot: | P05412 |
| Molecular weight: | 35kDa |
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