Western blot analysis of extracts of 293T cells, using Phospho-c-Jun-S63 antibody (AP1190) at 1:1000 dilution.293T cells were treated by Anisomycin (25 ug/ml) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-c-Jun-S63 Rabbit pAb
Catalog No:AB-AP1190
Manufacturer:ABclonal
Synonyms:AP-1;AP1;c-Jun;JUN;c
Applications:WB,IHC
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€150,00 – €410,00
Product Name: | Phospho-c-Jun-S63 Rabbit pAb |
Category: | Phosphorylated Antibodies |
Description: | This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
Immunogen: | A synthetic phosphorylated peptide around S63 of human Jun. |
Gene ID: | 3725 |
Swiss prot: | P05412 |
Molecular weight: | 35kDa |
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