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Western blot analysis of extracts of NIH/3T3, using Phospho-C/EBPB-T235 antibody (AP1055) at 1:1000 dilution.NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.NIH/3T3 cells were treated by Serum-starvation overnight at 37℃.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
Phospho-C/EBPB-T235 Rabbit pAb
Catalog No：AB-AP1055
Manufacturer：ABclonal
Synonyms：C/EBP-beta;IL6DBP;NF-IL6;TCF5;CEBP beta;CEBPB
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-C/EBPB-T235 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain. The encoded protein functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T235 of human C/EBPB. |
| Gene ID: | 1051 |
| Swiss prot: | P17676 |
| Molecular weight: | 36kDa |
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