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Western blot analysis of extracts of HeLa cells, using Phospho-BRD4-S498/S499 antibody (AP1179) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 180s.
Phospho-BRD4-S498/S499 Rabbit pAb
Catalog No：AB-AP1179
Manufacturer：ABclonal
Synonyms：BRD4; CAP; HUNK1; HUNKI; MCAP; bromodomain containing 4
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-BRD4-S498/S499 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding S498/S499 of Human Phospho-BRD4-S498/S499. |
| Gene ID: | 23476 |
| Swiss prot: | O60885 |
| Molecular weight: | 80kDa/88kDa/152kDa |
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