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Western blot analysis of extracts of various cell lines, using Phospho-Beclin 1-Ser15 antibody (AP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-Beclin 1-Ser15 Rabbit pAb
Catalog No：AB-AP1252
Manufacturer：ABclonal
Synonyms：ATG6;VPS30;beclin1;Beclin 1;BECN1;beclin-1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€150,00 – €410,00
| Product Name: | Phospho-Beclin 1-Ser15 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Ser15 of human Beclin 1 |
| Gene ID: | 8678 |
| Swiss prot: | Q14457 |
| Molecular weight: | 52kDa |
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