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Western blot analysis of extracts of HeLa cells, using Phospho-BCAR1-Y410 pAb (AP1058) at 1:1000 dilution or BCAR1 antibody (A18270).HeLa cells were treated by Pervanadate (1 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Phospho-BCAR1-Y410 Rabbit pAb
Catalog No：AB-AP1058
Manufacturer：ABclonal
Synonyms：BCAR1;CAS;CAS1;CASS1;CRKAS;P130Cas
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-BCAR1-Y410 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the Crk-associated substrate (CAS) family of scaffold proteins, characterized by the presence of multiple protein-protein interaction domains and many serine and tyrosine phosphorylation sites. The encoded protein contains a Src-homology 3 (SH3) domain, a proline-rich domain, a substrate domain which contains 15 repeat of the YxxP consensus phosphorylation motif for Src family kinases, a serine-rich domain, and a bipartite Src-binding domain, which can bind both SH2 and SH3 domains. This adaptor protein functions in multiple cellular pathways, including in cell motility, apoptosis and cell cycle control. Dysregulation of this gene can have a wide range of effects, affecting different pathways, including cardiac development, vascular smooth muscle cells, liver and kidney function, endothelial migration, and cancer. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding Y410 of human BCAR1. |
| Gene ID: | 9564 |
| Swiss prot: | P56945 |
| Molecular weight: | 93kDa |
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