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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Bad-S112 Rabbit mAb (AP1010) at 1:1000 dilution.NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight or treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 3min.
Phospho-Bad-S112 Rabbit mAb
Catalog No：AB-AP1010
Manufacturer：ABclonal
Synonyms：BBC2, BCL2L8
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-Bad-S112 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S112 of human Bad |
| Gene ID: | 572 |
| Swiss prot: | Q92934 |
| Molecular weight: | 23kDa |
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