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Western blot analysis of extracts of C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (AP1041) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-B-Raf-T401 Rabbit mAb
Catalog No：AB-AP1041
Manufacturer：ABclonal
Synonyms：B-RAF1, B-raf, BRAF1, NS7, RAFB1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €525,00
| Product Name: | Phospho-B-Raf-T401 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T401 of human B Raf |
| Gene ID: | 673 |
| Swiss prot: | P15056 |
| Molecular weight: | 85kDa |
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