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Western blot analysis of extracts of Raji cells, using Phospho-B-Raf-S445 pAb (AP0012) at 1:2000 dilution or BRAF antibody (A0038).Raji cells were treated by PMA/TPA (200nM) for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
Phospho-B-Raf-S445 Rabbit pAb
Catalog No：AB-AP0012
Manufacturer：ABclonal
Synonyms：BRAF;B-RAF1;B-raf;BRAF1;NS7;RAFB1
Applications：WB
Reactivity：Human,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-B-Raf-S445 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S445 of human BRAF (NP_004324.2). |
| Gene ID: | 673 |
| Swiss prot: | P15056 |
| Molecular weight: | 84kDa |
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