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Western blot analysis of extracts of various cell lines, using Phospho-AMPKa1/AMPKa2-T183/T172 antibody (AP1171) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C2C12 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.C6 cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Enhanced Kit (RM00021).|Exposure time: 60s.
Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb
Catalog No：AB-AP1171
Manufacturer：ABclonal
Synonyms：AMPKa1/AMPKa2
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | AMPK belongs to the ser/thr protein kinase family. Alpha subunit is the catalytic subunit of the 5′-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. |
| Immunogen: | A phospho synthetic peptide corresponding to residues surrounding T183/T172 of human AMPKa1/AMPKa2. |
| Gene ID: | 5562/5563 |
| Swiss prot: | Q13131/P54646 |
| Molecular weight: | 64kDa/65kDa/62kDa |
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