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Western blot analysis of extracts of 293T cells, using Phospho-AMPKɑ1-S485 antibody (AP0871) at 1:1000 dilution.293T cells were treated by oligomycin (0.5 uM) at 37℃ for 30 minutes.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
Phospho-AMPKα1-S485 Rabbit pAb
Catalog No：AB-AP0871
Manufacturer：ABclonal
Synonyms：PRKAA1;AMPK;AMPKa1
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-AMPK伪1-S485 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S485 of human PRKAA1 |
| Gene ID: | 5562 |
| Swiss prot: | Q13131 |
| Molecular weight: | 64kDa/65kDa |
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