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Western blot analysis of extracts of HT-29 cells, using Phospho-ALOX5-S271 antibody (AP0573) at 1:1000 dilution. HT-29 cells were treated by PMA/TPA (200nM).|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.
Phospho-ALOX5-S271 Rabbit pAb
Catalog No：AB-AP0573
Manufacturer：ABclonal
Synonyms：ALOX5;5-LO;5-LOX;5LPG;LOG5
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ALOX5-S271 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S271 of human ALOX5 (NP_000689.1). |
| Gene ID: | 240 |
| Swiss prot: | P09917 |
| Molecular weight: | 56kDa/61kDa/71kDa/74kDa/77kDa |
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