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Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1/PRAS40-T246 antibody (AP0793) at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
Phospho-AKT1S1/PRAS40-T246 Rabbit pAb
Catalog No：AB-AP0793
Manufacturer：ABclonal
Synonyms：AKT1S1;Lobe;PRAS40
Applications：WB
Reactivity：Human,Mouse
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-AKT1S1/PRAS40-T246 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008] |
| Immunogen: | A synthetic phosphorylated peptide around T246 of human AKT1S1/PRAS40 (NP_001092102.1). |
| Gene ID: | 84335 |
| Swiss prot: | Q96B36 |
| Molecular weight: | 13kDa/27kDa/29kDa |
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