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Western blot analysis of extracts of 293T cells, using Phospho-ACC1-S79 antibody (AP0298) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Phospho-ACC1-S79 Rabbit pAb
Catalog No：AB-AP0298
Manufacturer：ABclonal
Synonyms：ACACA;ACAC;ACACAD;ACC;ACC1;ACCA
Applications：WB
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €410,00
| Product Name: | Phospho-ACC1-S79 Rabbit pAb |
| Category: | Phosphorylated Antibodies |
| Description: | Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5′ sequence and encoding distinct isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S79 of human ACC1 |
| Gene ID: | 31 |
| Swiss prot: | Q13085 |
| Molecular weight: | 257kDa/259kDa/265kDa/269kDa |
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