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Western blot analysis of extracts of 293T cells, using Phospho-β-Catenin-S33/S37 Rabbit mAb (AP0979) at 1:1000 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit (RM00020).|Exposure time: 3min.
Phospho-β-Catenin-S33/S37 Rabbit mAb
Catalog No：AB-AP0979
Manufacturer：ABclonal
Synonyms：CTNNB, MRD19, armadillo
Applications：WB
Reactivity：Human
Host：Rabbit
Conjugate：No
€65,00 – €325,00
| Product Name: | Phospho-尾-Catenin-S33/S37 Rabbit mAb |
| Category: | Phosphorylated Antibodies |
| Description: | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016] |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S33/S37 of human beta Catenin |
| Gene ID: | 1499 |
| Swiss prot: | P35222 |
| Molecular weight: | 92kDa |
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