Western blot analysis of extracts of various cell lines, using PHLPP1 antibody (A9542) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
PHLPP1 Rabbit pAb
Catalog No:AB-A9542
Manufacturer:ABclonal
Synonyms:PHLPP1;PHLPP;PLEKHE1;PPM3A;SCOP
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PHLPP1 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1508-1717 of human PHLPP1 (NP_919431.2). |
Gene ID: | 23239 |
Swiss prot: | O60346 |
Molecular weight: | 133kDa/184kDa |
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