Western blot analysis of extracts of HeLa cells, using PHF8 antibody (A19780) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
Western blot analysis of extracts of various cell lines, using PHF8 antibody (A19780) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 180s.
PHF8 Rabbit mAb
Catalog No:AB-A19780
Manufacturer:ABclonal
Synonyms:JHDM1F, KDM7B, MRXSSD, ZNF422
Applications:WB
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €485,00
Product Name: | PHF8 Rabbit mAb |
Category: | Monoclonal Antibodies |
Description: | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. Defects in this gene are a cause of mental retardation syndromic X-linked Siderius type (MRXSSD). Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010] |
Immunogen: | A synthesized peptide derived from human PHF8 |
Gene ID: | 23133 |
Swiss prot: | Q9UPP1 |
Molecular weight: | 140kDa |
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