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Western blot analysis of extracts of various cell lines, using PFKM antibody (A5477) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human esophageal cancer using PFKM antibody (A5477) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using PFKM antibody (A5477) at dilution of 1:100 (40x lens).
PFKM Rabbit pAb
Catalog No：AB-A5477
Manufacturer：ABclonal
Synonyms：PFKM;ATP-PFK;GSD7;PFK-1;PFK1;PFKA;PFKX;PPP1R122
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | PFKM Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human PFKM (NP_001160160.1). |
| Gene ID: | 5213 |
| Swiss prot: | P08237 |
| Molecular weight: | 81kDa/85kDa/93kDa |
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