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Western blot analysis of extracts of various cell lines, using Perforin antibody (A0093) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody (A0093) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody (A0093) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH/3T3 cells using Perforin antibody (A0093) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody (A0093) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody (A0093) at dilution of 1:100 (40x lens).
Perforin Rabbit pAb
Catalog No：AB-A0093
Manufacturer：ABclonal
Synonyms：PRF1;FLH2;HPLH2;P1;PFN1;PFP;perforin-1;Perforin
Applications：WB,IHC,IF
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Perforin Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1). |
| Gene ID: | 5551 |
| Swiss prot: | P14222 |
| Molecular weight: | 61kDa |
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