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Western blot analysis of extracts of various cell lines, using Pepsinogen II antibody (A2788) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 5s.
Pepsinogen II Rabbit pAb
Catalog No：AB-A2788
Manufacturer：ABclonal
Synonyms：PGC;PEPC;PGII;gastricsin
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €370,00
| Product Name: | Pepsinogen II Rabbit pAb |
| Category: | Polyclonal Antibodies |
| Description: | This gene encodes an aspartic proteinase that belongs to the peptidase family A1. The encoded protein is a digestive enzyme that is produced in the stomach and constitutes a major component of the gastric mucosa. This protein is also secreted into the serum. This protein is synthesized as an inactive zymogen that includes a highly basic prosegment. This enzyme is converted into its active mature form at low pH by sequential cleavage of the prosegment that is carried out by the enzyme itself. Polymorphisms in this gene are associated with susceptibility to gastric cancers. Serum levels of this enzyme are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Pepsinogen II (NP_001159896.1). |
| Gene ID: | 5225 |
| Swiss prot: | P20142 |
| Molecular weight: | 34kDa/42kDa |
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