Western blot analysis of extracts of various cell lines, using PDK4 antibody (A13337) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 1s.
PDK4 Rabbit pAb
Catalog No:AB-A13337
Manufacturer:ABclonal
Synonyms:PDK4
Applications:WB,IHC,IF
Reactivity:Human,Mouse,Rat
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PDK4 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 102-411 of human PDK4 (NP_002603.1). |
Gene ID: | 5166 |
Swiss prot: | Q16654 |
Molecular weight: | 46kDa |
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