Western blot analysis of extracts of various cell lines, using PDK2 antibody (A4012) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 90s.
PDK2 Rabbit pAb
Catalog No:AB-A4012
Manufacturer:ABclonal
Synonyms:PDK2;PDHK2;PDKII
Applications:WB,IF
Reactivity:Human
Host:Rabbit
Conjugate:No
€65,00 – €370,00
Product Name: | PDK2 Rabbit pAb |
Category: | Polyclonal Antibodies |
Description: | This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 128-407 of human PDK2 (NP_002602.2). |
Gene ID: | 5164 |
Swiss prot: | Q15119 |
Molecular weight: | 38kDa/46kDa |
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