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Western blot analysis of extracts of various cell lines, using Pyruvate Dehydrogenase E1 beta subunit Rabbit mAb (A4645) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit (RM00020).|Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded rat kidney using PDHB Rabbit mAb (A4645) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using PDHB Rabbit mAb (A4645) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using PDHB Rabbit mAb (A4645) at dilution of 1:100 (40x lens).
PDHB Rabbit mAb
Catalog No：AB-A4645
Manufacturer：ABclonal
Synonyms：PDHBD, PDHE1-B, PHE1B
Applications：WB,IHC
Reactivity：Human,Mouse,Rat
Host：Rabbit
Conjugate：No
€65,00 – €485,00
| Product Name: | PDHB Rabbit mAb |
| Category: | Monoclonal Antibodies |
| Description: | The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and carbon dioxide, and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 beta subunit. Mutations in this gene are associated with pyruvate dehydrogenase E1-beta deficiency. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2012] |
| Immunogen: | A synthesized peptide derived from human Pyruvate Dehydrogenase E1 beta subunit |
| Gene ID: | 5162 |
| Swiss prot: | P11177 |
| Molecular weight: | 35kDa |
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